Limiting dilution analysis, or limiting dilution assay, or dilution cloning. An older technique of obtaining a monoclonal cell culture from a heterogenous population of cells by setting up a series of dilutions from cell suspensions.
Found 2 search result(s) for "limiting dilution".
8.2 David Ho — Unraveling of HIV Dynamics In Vivo
(HIV/AIDS Research: Its History & Future Meeting)
... did a systematic evaluation of trying to quantify HIV in blood. This we did by the oldfashioned limiting dilution culture technique. (1) To our surprise, we found that in a lot of cases, a few ...
Apr 27, 2021
... did a systematic evaluation of trying to quantify HIV in blood. This we did by the oldfashioned limiting dilution culture technique. (1) To our surprise, we found that in a lot of cases, a few ...
Apr 27, 2021
8.4 Robert Siliciano — The Challenge of the HIV Reservoir
(HIV/AIDS Research: Its History & Future Meeting)
... definitive assay for this reservoir. In this assay, you take resting cells, plate them in limiting dilution, and then activate them under conditions where 100% of the cells undergo blast transformation ...
Apr 27, 2021
... definitive assay for this reservoir. In this assay, you take resting cells, plate them in limiting dilution, and then activate them under conditions where 100% of the cells undergo blast transformation ...
Apr 27, 2021